๐ก This study investigates the gut microbiome’s role in the carriage of ๐๐ด๐ค๐ฉ๐ฆ๐ณ๐ช๐ค๐ฉ๐ช๐ข ๐ค๐ฐ๐ญ๐ช sequence type 131-H30R (ST131-H30R), a global multidrug-resistant pathogen. Through longitudinal fecal swab surveillance, they characterized the fecal microbiome of veterans and their household members, aiming to identify associations between specific gut bacterial phyla and genera and ST131-H30R carriage.
๐๐ด๐ค๐ฉ๐ฆ๐ณ๐ช๐ค๐ฉ๐ช๐ข ๐ค๐ฐ๐ญ๐ช sequence type 131-H30R (ST131-H30R) is a global multidrug-resistant pathogen, yet the role of the gut microbiome in its intestinal carriage remains unclear. This study aims to characterize the associations between specific gut bacterial phyla and genera and the carriage, persistence, and loss of ST131-H30R, providing potential targets for interventions.
๐ Methods:
Participants, consisting of veterans and their household members, underwent longitudinal fecal swab surveillance from 2014 to 2018. Fecal microbiome characterization involved 16S rRNA qPCR and sequencing. Associations between ST131-H30R carriage and the gut microbiome were assessed through random forest models at baseline and mixed-effects logistic regression in longitudinal analyses.
๐ Key Findings:
๐ Baseline Associations: H30R-positive participants exhibited higher proportional abundances of ๐๐ค๐ต๐ช๐ฏ๐ฐ๐ฃ๐ข๐ค๐ต๐ฆ๐ณ๐ช๐ข phylum, specifically 4.9% vs. 3.1% in ST131-negative participants. ๐๐ฐ๐ญ๐ญ๐ช๐ฏ๐ด๐ฆ๐ญ๐ญ๐ข abundance was higher (2.3% vs. 1.1%), while ๐๐ญ๐ช๐ด๐ต๐ช๐ฑ๐ฆ๐ด abundance was lower (2.1% vs. 2.6%) in H30R-positive participants.
๐ Longitudinal Associations: ๐๐ฐ๐ญ๐ญ๐ช๐ฏ๐ด๐ฆ๐ญ๐ญ๐ข abundance correlated positively with ST131-H30R carriage status and negatively with the loss of ST131-H30R.
๐๐ญ๐ช๐ด๐ต๐ช๐ฑ๐ฆ๐ด corresponded with the loss and persistent absence of ST131-H30R, even in the presence of household exposure.
๐ Potential Intervention Targets: ๐๐ฐ๐ญ๐ญ๐ช๐ฏ๐ด๐ฆ๐ญ๐ญ๐ข, specifically ๐. ๐ข๐ฆ๐ณ๐ฐ๐ง๐ข๐ค๐ช๐ฆ๐ฏ๐ด, associated with ST131 and H30R presence, suggesting it as a potential intervention target. ๐๐ญ๐ช๐ด๐ต๐ช๐ฑ๐ฆ๐ด, negatively associated with H30R prevalence, may be a target for reducing ST131-H30R colonization.
๐ ๐๐ฐ๐ญ๐ญ๐ช๐ฏ๐ด๐ฆ๐ญ๐ญ๐ข, associated with various diseases, demonstrated positive correlations with ST131-H30R, suggesting its potential as an intervention target. In contrast, ๐๐ญ๐ช๐ด๐ต๐ช๐ฑ๐ฆ๐ด, known for anti-inflammatory effects, showed negative associations with H30R, offering another potential target. Future investigations should explore these associations’ causal mechanisms and evaluate interventions, including probiotics and diet modifications.
๐ This study unveils associations between specific gut microbiome components and ST131-H30R carriage, persistence, and loss. ๐๐ฐ๐ญ๐ญ๐ช๐ฏ๐ด๐ฆ๐ญ๐ญ๐ข and ๐๐ญ๐ช๐ด๐ต๐ช๐ฑ๐ฆ๐ด emerge as potential intervention targets, offering avenues for microbiome-based strategies to mitigate the risk of ST131-H30R, a significant multidrug-resistant pathogen.
Link to the article : http://tinyurl.com/4zz86bmp
