๐ก This scientific report presents a comprehensive study focused on profiling drug combinations against Gram-positive bacterial species, including ๐๐ข๐ค๐ช๐ญ๐ญ๐ถ๐ด ๐ด๐ถ๐ฃ๐ต๐ช๐ญ๐ช๐ด, ๐๐ต๐ข๐ฑ๐ฉ๐บ๐ญ๐ฐ๐ค๐ฐ๐ค๐ค๐ถ๐ด ๐ข๐ถ๐ณ๐ฆ๐ถ๐ด, ๐ข๐ฏ๐ฅ ๐๐ต๐ณ๐ฆ๐ฑ๐ต๐ฐ๐ค๐ฐ๐ค๐ค๐ถ๐ด ๐ฑ๐ฏ๐ฆ๐ถ๐ฎ๐ฐ๐ฏ๐ช๐ข๐ฆ.
๐ The research tested approximately 8,000 combinations of 65 antibacterial drugs, including commonly used antibiotics, neglected antibiotics, and non-antibiotic drugs with reported antibacterial activity.
๐ The study identifies numerous synergies and antagonisms, providing a valuable resource for understanding drug interactions and improving antimicrobial therapies.
๐ Methods:
Study Focus: Profiling drug combinations against ๐๐ข๐ค๐ช๐ญ๐ญ๐ถ๐ด ๐ด๐ถ๐ฃ๐ต๐ช๐ญ๐ช๐ด, ๐๐ต๐ข๐ฑ๐ฉ๐บ๐ญ๐ฐ๐ค๐ฐ๐ค๐ค๐ถ๐ด ๐ข๐ถ๐ณ๐ฆ๐ถ๐ด, ๐ข๐ฏ๐ฅ ๐๐ต๐ณ๐ฆ๐ฑ๐ต๐ฐ๐ค๐ฐ๐ค๐ค๐ถ๐ด ๐ฑ๐ฏ๐ฆ๐ถ๐ฎ๐ฐ๐ฏ๐ช๐ข๐ฆ.
Compound Selection: Approximately 8,000 combinations involving 65 antibacterial drugs, including antibiotics from various classes, commonly prescribed non-antibiotic drugs, and antibiotic adjuvants.
Dose-Dependent Analysis: Interactions evaluated in a quantitative manner.
Synergy and Antagonism Identification: Quantification of interactions based on growth inhibition, killing, and clearing of infections.
๐ Key Findings:
Synergies in Gram-Positive Bacteria: Identified numerous synergistic interactions, some of which were effective against multidrug-resistant clinical isolates. Synergies often species-specific, influenced by cell surface characteristics and drug uptake differences.
Antagonisms and Non-Antibiotic Drugs: Uncovered antagonistic interactions between commonly administered non-antibiotic drugs and antibiotics, potentially impacting antibiotic therapy efficacy.
Specific Case: Ticagrelor: Focused on the anti-aggregant ticagrelor, revealing 13 additional synergies with antibiotics in Staphylococcus aureus.
Provided insights into how ticagrelor affects bacterial physiology and enhances the action of positively charged antibiotics.
Conservation of Interactions: Interactions generally species-specific, with synergies tending to be more conserved than antagonisms. Differences in cell surface organization and drug permeability explained variation in interaction profiles.
๐ This systematic profiling of drug combinations against Gram-positive bacteria provides a wealth of information on synergies and antagonisms, shedding light on novel approaches to combat bacterial infections. The study emphasizes the importance of assessing drug interactions not only in growth inhibition but also in the context of bacterial killing and infection clearance.
Link to the article : https://tinyurl.com/2p8874j3
