Resveratrol-Loaded Liposomes- A Novel Approach to Enhance Antibacterial Activity and Biofilm Disruption in 𝘚. 𝘢𝘶𝘳𝘦𝘶𝘴 𝘢𝘯𝘥 𝘔𝘙𝘚𝘈

💡 Research on the antimicrobial and anti-adherence effects of Resveratrol, a natural product, is a focus of growing interest to combat the rising trend of antimicrobial resistance. 𝘚𝘵𝘢𝘱𝘩𝘺𝘭𝘰𝘤𝘰𝘤𝘤𝘶𝘴 𝘢𝘶𝘳𝘦𝘶𝘴 𝘢𝘯𝘥 𝘔𝘦𝘵𝘩𝘪𝘤𝘪𝘭𝘭𝘪𝘯-𝘳𝘦𝘴𝘪𝘴𝘵𝘢𝘯𝘵 𝘚. 𝘢𝘶𝘳𝘦𝘶𝘴 are bacteria of particular concern and are the focus of this paper.  

📍 The study aims to investigate the antibacterial and anti-biofilm activity of Resveratrol on 𝘚𝘵𝘢𝘱𝘩𝘺𝘭𝘰𝘤𝘰𝘤𝘤𝘶𝘴 𝘢𝘶𝘳𝘦𝘶𝘴 𝘢𝘯𝘥 𝘔𝘦𝘵𝘩𝘪𝘤𝘪𝘭𝘭𝘪𝘯-𝘳𝘦𝘴𝘪𝘴𝘵𝘢𝘯𝘵 𝘚. 𝘢𝘶𝘳𝘦𝘶𝘴 strains, study the added value of delivering RSV through liposomes on its antimicrobial properties, and analyze the anti adherence effects of RSV when it is embedded in liposomes on the bacterial strains. 

📌 The study involved formulating liposomes to entrap Resveratrol, evaluating their interaction with bacteria, and assessing their in vitro release. The antibacterial, anti-biofilm, and anti-adherence activities of free and liposome-embedded Resveratrol against 𝘚𝘵𝘢𝘱𝘩𝘺𝘭𝘰𝘤𝘰𝘤𝘤𝘶𝘴 𝘢𝘶𝘳𝘦𝘶𝘴 𝘢𝘯𝘥 𝘔𝘦𝘵𝘩𝘪𝘤𝘪𝘭𝘭𝘪𝘯-𝘳𝘦𝘴𝘪𝘴𝘵𝘢𝘯𝘵 𝘚. 𝘢𝘶𝘳𝘦𝘶𝘴 were then evaluated. 

1. Resveratrol was found to have the potential to damage the bacterial cell wall, resulting in observable breaks or pores. 

2. Both free Resveratrol and Resveratrol loaded into liposomes demonstrated bacteriostatic activity against 𝘚𝘵𝘢𝘱𝘩𝘺𝘭𝘰𝘤𝘰𝘤𝘤𝘶𝘴 𝘢𝘶𝘳𝘦𝘶𝘴 𝘢𝘯𝘥 𝘔𝘦𝘵𝘩𝘪𝘤𝘪𝘭𝘭𝘪𝘯-𝘳𝘦𝘴𝘪𝘴𝘵𝘢𝘯𝘵 𝘚. 𝘢𝘶𝘳𝘦𝘶𝘴. 

3. The loaded Resveratrol in the liposomes functionalized with GLT1 greatly increased the anti-adhesive properties and also fully inhibited bacteriostatic activity. 

4. There was observed interaction between the galactosylated liposomes and the bacterial surface, showing differential interactions with the surfaces. 

5. When transferred to a fresh medium, Resveratrol-treated bacteria were able to grow, suggesting that the cell wall damage might not be permanent, but a reversible or transient phenomenon. 

📍 The paper concludes that while RSV has antimicrobial and anti-adherence effects, its poor solubility in biological fluids presents a challenge. However, the successful encapsulation of RSV within liposomes has improved its bioavailability, thus enhancing its therapeutic potential. Further investigations are required to understand RSV’s mechanism of action and its use as an adjuvant in biofilm-associated antibacterial therapies. 

Resveratrol supports antibiotic stewardship programs by demonstrating antibacterial and anti-biofilm effects, particularly against 𝘚𝘵𝘢𝘱𝘩𝘺𝘭𝘰𝘤𝘰𝘤𝘤𝘶𝘴 𝘢𝘶𝘳𝘦𝘶𝘴 𝘢𝘯𝘥 𝘔𝘦𝘵𝘩𝘪𝘤𝘪𝘭𝘭𝘪𝘯-𝘳𝘦𝘴𝘪𝘴𝘵𝘢𝘯𝘵 𝘚. 𝘢𝘶𝘳𝘦𝘶𝘴. Its use could potentially lower reliance on traditional antibiotics, thus decreasing the risk of resistance. Used with conventional antibiotics or as an adjuvant, RSV could enhance treatment effectiveness, potentially allowing dose reduction. However, careful use is needed to prevent resistance due to overuse or misuse. 

Link to the article: http://tinyurl.com/yc5p24hs