๐ก This study investigates the impact of acute high-altitude hypoxia on gut microbiota and intestinal structure in SpragueโDawley rats and examines the potential protective effects of oxygen enrichment.
๐ The results demonstrate that acute hypoxia leads to intestinal injury, disrupting the morphological structure of the gut and altering gut flora.
Notably, oxygen enrichment significantly mitigates intestinal damage and restores the gut microbiotaโs richness, diversity, and composition, indicating its potential as a therapeutic intervention at high altitudes.
๐ Methods:
๐ Eighteen male SpragueโDawley rats were divided into three groups: control (NN), hypobaric hypoxic (HH), and oxygen-enriched (HO). The HH group was exposed to simulated high-altitude hypoxia (7,000 m) for 3 days, while the HO group received oxygen enrichment for 12 hours daily.
Gut microbiota and intestinal structure were analyzed using high-throughput 16S rRNA gene sequencing and morphological assessments.
โญ Key Findings:
๐ Acute high-altitude hypoxia induced severe structural damage to the colon tissue, including a reduction in crypt depth, epithelial cell shedding, and decreased goblet cell count, indicating intestinal injury.
๐ Gut microbiota composition analysis revealed an altered ratio of Firmicutes to Bacteroidetes and changes in specific genera like ๐๐ข๐ค๐ต๐ฐ๐ฃ๐ข๐ค๐ช๐ญ๐ญ๐ถ๐ด ๐ข๐ฏ๐ฅ ๐๐ณ๐ฆ๐ท๐ฐ๐ต๐ฆ๐ญ๐ญ๐ข๐ค๐ฆ๐ข๐ฆ๐๐3๐31 group in response to hypoxia.
๐ Oxygen enrichment significantly mitigated intestinal injury, as evidenced by increased crypt depth, intact epithelial cell morphology, and enhanced goblet cell density compared to the HH group.
๐ Oxygen enrichment also positively influenced gut microbiota, promoting higher richness, evenness, and diversity. It restored the balance of Bacteroidetes and Prevotellaceae, crucial microbiota at high altitudes.
Functional analysis indicated that oxygen enrichment affected metabolic pathways related to energy metabolism, amino acid metabolism, and the tricarboxylic acid cycle, potentially aiding in metabolic resilience during hypoxia.
๐ This study demonstrates the protective effects of oxygen enrichment on gut microbiota and intestinal structure in response to acute high-altitude hypoxia. Oxygen enrichment mitigates intestinal injury and helps restore gut microbiota richness and diversity, highlighting its potential as a therapeutic intervention to counteract hypoxic damage at high altitudes.
Link to the article: https://tinyurl.com/259v3nxf
