๐ก The paper explores the effect of cell-free nontuberculous ๐๐บ๐ค๐ฐ๐ฃ๐ข๐ค๐ต๐ฆ๐ณ๐ช๐ถ๐ฎ supernatants on the antibiotic resistance and biofilm-forming abilities of opportunistic pathogens, specifically ๐๐ด๐ค๐ฉ๐ฆ๐ณ๐ช๐ค๐ฉ๐ช๐ข ๐ค๐ฐ๐ญ๐ช ๐ข๐ฏ๐ฅ ๐๐ต๐ข๐ฑ๐ฉ๐บ๐ญ๐ฐ๐ค๐ฐ๐ค๐ค๐ถ๐ด ๐ฆ๐ฑ๐ช๐ฅ๐ฆ๐ณ๐ฎ๐ช๐ฅ๐ช๐ด. The researchers aimed to investigate how these supernatants may influence the pathogens’ susceptibility to antibiotics and capabilities to form biofilms.
๐ The study used strains of ๐๐ด๐ค๐ฉ๐ฆ๐ณ๐ช๐ค๐ฉ๐ช๐ข ๐ค๐ฐ๐ญ๐ช ๐ข๐ฏ๐ฅ ๐๐ต๐ข๐ฑ๐ฉ๐บ๐ญ๐ฐ๐ค๐ฐ๐ค๐ค๐ถ๐ด ๐ฆ๐ฑ๐ช๐ฅ๐ฆ๐ณ๐ฎ๐ช๐ฅ๐ช๐ด with differing anti-hemoglobin activity. Supernatants from ๐๐บ๐ค๐ฐ๐ญ๐ช๐ค๐ช๐ฃ๐ข๐ค๐ต๐ฆ๐ณ๐ช๐ถ๐ฎ ๐ช๐ณ๐ข๐ฏ๐ช๐ค๐ถ๐ฎ (IKVS S1 and IKVS S2) were acquired through cultivation in broth, centrifugation, and filtration. The susceptibility of the pathogens to antibiotics was measured with the KirbyโBauer disk diffusion method, and their biofilm formation intensity upon exposure to the supernatants was determined using crystal violet staining.
๐ Exposure to cell-free ๐๐บ๐ค๐ฐ๐ญ๐ช๐ค๐ช๐ฃ๐ข๐ค๐ต๐ฆ๐ณ๐ช๐ถ๐ฎ ๐ช๐ณ๐ข๐ฏ๐ช๐ค๐ถ๐ฎ supernatants resulted in a significant increase in biofilm formation in both ๐. ๐ค๐ฐ๐ญ๐ช ๐ข๐ฏ๐ฅ ๐. ๐ฆ๐ฑ๐ช๐ฅ๐ฆ๐ณ๐ฎ๐ช๐ฅ๐ช๐ด.
The most pronounced enhancement in biofilm formation was observed under the influence of the ๐๐บ๐ค๐ฐ๐ญ๐ช๐ค๐ช๐ฃ๐ข๐ค๐ต๐ฆ๐ณ๐ช๐ถ๐ฎ ๐ช๐ณ๐ข๐ฏ๐ช๐ค๐ถ๐ฎ IKVS S1 supernatant.
The ๐. ๐ค๐ฐ๐ญ๐ช ๐ข๐ฏ๐ฅ ๐. ๐ฆ๐ฑ๐ช๐ฅ๐ฆ๐ณ๐ฎ๐ช๐ฅ๐ช๐ด. bacteria strains showed a notable decrease in susceptibility to various antibiotics when exposed to the ๐. ๐ช๐ณ๐ข๐ฏ๐ช๐ค๐ถ๐ฎ supernatants.
The intensification in biofilm formation and decrease in antibiotic susceptibility (vancomycin and cefotaxime) was particularly evident in strains showing higher levels of anti-hemoglobin activity.
The presence of a potential synergistic relationship between ๐. ๐ช๐ณ๐ข๐ฏ๐ช๐ค๐ถ๐ฎ and ๐. ๐ค๐ฐ๐ญ๐ช ๐ข๐ฏ๐ฅ ๐. ๐ฆ๐ฑ๐ช๐ฅ๐ฆ๐ณ๐ฎ๐ช๐ฅ๐ช๐ด was suggested, which could impact their interaction with each other and their overall biological behaviors.
๐ The increased biofilm formation and heightened antibiotic resistance upon exposure to ๐. ๐ช๐ณ๐ข๐ฏ๐ช๐ค๐ถ๐ฎ, as identified in this study, could potentially impact the virulence of these opportunistic bacteria strains. This also suggests that cell-free supernatants of certain bacteria could influence the severity and treatment outcomes of infections caused by opportunistic pathogens like ๐. ๐ค๐ฐ๐ญ๐ช ๐ข๐ฏ๐ฅ ๐. ๐ฆ๐ฑ๐ช๐ฅ๐ฆ๐ณ๐ฎ๐ช๐ฅ๐ช๐ด. This research could have potential implications in understanding disease progression and optimising treatment strategies for bacterial infections.ย
Link to the article : https://tinyurl.com/2jtn927n
