📍 Antimicrobial peptidomimetics are synthetic compounds designed to mimic natural peptides, targeting bacterial cell membranes to prevent the development of bacterial resistance.
💡 The study aims to assess the ability of antimicrobial peptidomimetics to prevent bacterial resistance towards two commonly used antibiotics, Gentamicin and Ciprofloxacin, in 𝘚𝘵𝘢𝘱𝘩𝘺𝘭𝘰𝘤𝘰𝘤𝘤𝘶𝘴 𝘢𝘯𝘥 𝘗𝘴𝘦𝘶𝘥𝘰𝘮𝘰𝘯𝘢𝘴 bacteria over time.
📌 The Minimum Inhibitory Concentration and Fractional Inhibitory Concentration of antibiotics and peptidomimetics were determined using methods such as the checkerboard microtiter broth dilution method.
📌 Bacteria were passaged daily in the presence of antibiotics or a combination of antibiotics and peptidomimetics at certain concentrations. The MICs and FICs were tested daily and compared after 10 and 30-day periods.
📍 The research identified that 𝘚𝘵𝘢𝘱𝘩𝘺𝘭𝘰𝘤𝘰𝘤𝘤𝘶𝘴 𝘢𝘯𝘥 𝘗𝘴𝘦𝘶𝘥𝘰𝘮𝘰𝘯𝘢𝘴 bacteria developed resistance towards Gentamicin and Ciprofloxacin antibiotics when repeatedly exposed to them under sub-inhibitory concentrations over 10 days.
📌 However, when these antibiotics were combined with peptidomimetics, such as Melimine and Mel4, the Minimum Inhibition Concentration did not change, indicating no development of resistance.
📌 The study also revealed that the use of a combination of Gentamicin and Ciprofloxacin resulted in a less significant increase in MICs compared to the use of a single antibiotic.
📌 The effect of peptidomimetics did not alter even after 30 days of testing, representing their sustained effectiveness over time.
📌 The was no cross-resistance, meaning the bacteria did not develop resistance against different types of antibiotics.
📍 The study concluded that combining antibiotics with antimicrobial peptidomimetics can mitigate the development of antibiotic resistance in both 𝘚𝘵𝘢𝘱𝘩𝘺𝘭𝘰𝘤𝘰𝘤𝘤𝘶𝘴 𝘢𝘯𝘥 𝘗𝘴𝘦𝘶𝘥𝘰𝘮𝘰𝘯𝘢𝘴 bacteria. It demonstrated the potential of peptidomimetics as effective antimicrobial adjuvants to reduce the rate of antibiotic resistance development and improve the effectiveness of current antibiotics.
Link to the article : https://tinyurl.com/4b3d7ak8
