𝐓𝐡𝐞 𝐩𝐨𝐭𝐞𝐧𝐭𝐢𝐚𝐥 𝐢𝐦𝐦𝐮𝐧𝐨𝐦𝐨𝐝𝐮𝐥𝐚𝐭𝐨𝐫𝐲 𝐫𝐨𝐥𝐞 𝐨𝐟 𝐭𝐡𝐞 𝐠𝐮𝐭 𝐦𝐢𝐜𝐫𝐨𝐛𝐢𝐨𝐭𝐚 𝐢𝐧 𝐭𝐡𝐞 𝐩𝐚𝐭𝐡𝐨𝐠𝐞𝐧𝐞𝐬𝐢𝐬 𝐨𝐟 𝐚𝐬𝐭𝐡𝐦𝐚

💡 This study aimed to investigate the impact of Bacteroides vulgatus (BV), Clostridium perfringens (CP), Parabacteroides distasonis (PD), and Ruminococcus albus (RA) lysates on the secretion of key cytokines (IL-1β, IL-6, IL-10, TNF-α) by human peripheral blood mononuclear cells (PBMC), monocyte-derived macrophages (MDM), and HT-29 cells.

📍 The study delves into potential mechanisms underlying their influence on asthma development. Enzyme-linked immunosorbent assays were employed for cytokine analysis.

📍 Methods:

The study assessed the impact of BV, CP, PD, and RA lysates on cytokine secretion by PBMC, MDM, and HT-29 cells. Various lysate doses (100 µg and 400 µg) were administered, and cytokine levels were measured using enzyme-linked immunosorbent assays.

📍 Key Findings:

📌 IL-1β Secretion: BV and CP lysates significantly reduced IL-1β secretion by MDM at 400 µg (p < 0.05 and p < 0.001 respectively). RA lysates at 100 µg slightly decreased IL-1β in MDM.

📌 IL-6 Secretion: CP and PD lysates significantly increased IL-6 secretion by PBMC and MDM (p < 0.05). Other lysates induced IL-6 secretion without statistical significance. HT-29 cells showed a non-significant decrease in IL-6.

📌 IL-10 Secretion: BV, CP, and PD lysates at 100 µg significantly increased IL-10 secretion by PBMC (p < 0.05). CP, PD, and RA lysates at 400 µg significantly increased IL-10 in MDM (p < 0.001). BV lysate at 400 µg increased IL-10 in MDM (p < 0.05).

📌 TNF-α Secretion: BV lysate at 100 µg and 400 µg significantly increased TNF-α secretion by MDM (p < 0.05 and p < 0.001 respectively). PD lysates at 400 µg increased TNF-α in MDM (p < 0.05).

📍 The study elucidates the intricate interactions between bacterial lysates and cytokine secretion in immune and epithelial cells, potentially contributing to asthma pathogenesis. BV and CP lysates demonstrated anti-inflammatory effects by lowering IL-1β, while CP and PD lysates induced pro-inflammatory IL-6 secretion.

Additionally, BV, CP, and PD lysates promoted anti-inflammatory IL-10 production, suggesting potential immunomodulatory roles in asthma.

Link to the article : https://tinyurl.com/pfsz84je