๐ก This study investigated the interactions between Colorectal cancer (CRC)-associated bacterial species (๐๐ฏ๐ต๐ฆ๐ณ๐ฐ๐ต๐ฐ๐น๐ช๐จ๐ฆ๐ฏ๐ช๐ค ๐๐ข๐ค๐ต๐ฆ๐ณ๐ฐ๐ช๐ฅ๐ฆ๐ด ๐ง๐ณ๐ข๐จ๐ช๐ญ๐ช๐ด, ๐๐ฏ๐ต๐ฆ๐ณ๐ฐ๐ค๐ฐ๐ค๐ค๐ถ๐ด ๐ง๐ข๐ฆ๐ค๐ข๐ญ๐ช๐ด, ๐ข๐ฏ๐ฅ ๐๐ถ๐ด๐ฐ๐ฃ๐ข๐ค๐ต๐ฆ๐ณ๐ช๐ถ๐ฎ ๐ฏ๐ถ๐ค๐ญ๐ฆ๐ข๐ต๐ถ๐ฎ) and a probiotic species (๐๐ด๐ค๐ฉ๐ฆ๐ณ๐ช๐ค๐ฉ๐ช๐ข ๐ค๐ฐ๐ญ๐ช ๐๐ช๐ด๐ด๐ญ๐ฆ 1917) with benign (S/RG/C2) and malignant (HCT116) colorectal tumour cell lines.
๐ The results shed light on potential tumour cell-specific bacterial effects and their implications in CRC progression.
๐ Methods:
๐ Gentamicin protection assays to measure bacterial attachment and invasion to tumour cell monolayers.
๐ Cell culture conditions to assess bacterial persistence within tumour cells.
๐Biofilm assays to study the influence of mucin on bacterial phenotype.
๐Scratch assays and transwell filter assays to analyze tumour cell migration and invasion upon bacterial treatment.
๐ Key Findings:
๐ All CRC-associated bacterial species demonstrated higher attachment to benign tumour cells (S/RG/C2) compared to malignant tumour cells (HCT116).
๐ Invasion rates were higher in HCT116 cells, suggesting their predisposition to bacterial invasion.
๐ Bacterial species persisted within tumour cells for at least 48 hours post-infection, with greater persistence in malignant cells.
๐ Bacterial treatment significantly increased tumour cell yield in both benign and malignant cell lines.
๐๐. ๐ฏ๐ถ๐ค๐ญ๐ฆ๐ข๐ต๐ถ๐ฎ notably increased migration and invasion potential of malignant cells (HCT116).
๐ The study highlights varying interactions of CRC-associated bacteria with benign and malignant tumour cells. The differential effects on migration and invasion emphasize potential tumour cell-specific bacterial influences.
Link to the article : https://tinyurl.com/msnyjdp9
