𝐏𝐨𝐭𝐞𝐧𝐭𝐢𝐚𝐥 𝐨𝐟 𝐁𝐚𝐜𝐢𝐥𝐥𝐮𝐬-𝐃𝐞𝐫𝐢𝐯𝐞𝐝 𝐀𝐧𝐭𝐢𝐦𝐢𝐜𝐫𝐨𝐛𝐢𝐚𝐥 𝐏𝐞𝐩𝐭𝐢𝐝𝐞𝐬 𝐚𝐬 𝐀𝐥𝐭𝐞𝐫𝐧𝐚𝐭𝐢𝐯𝐞𝐬 𝐭𝐨 𝐀𝐧𝐭𝐢𝐛𝐢𝐨𝐭𝐢𝐜𝐬 𝐢𝐧 𝐋𝐢𝐯𝐞𝐬𝐭𝐨𝐜𝐤 𝐈𝐧𝐝𝐮𝐬𝐭𝐫𝐲

💡 In this study, the authors are addressing the growing problem of antibiotic resistance due to the widespread use of antibiotics in the livestock industry. They’re looking at antimicrobial peptides produced by 𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴 species as an alternative to traditional antibiotics. 

📍 The researchers aim to isolate, identify, and examine the antimicrobial activity and stability of AMPs produced by two specific strains of

𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴—𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴 𝘷𝘦𝘭𝘦𝘻𝘦𝘯𝘴𝘪𝘴 9-1 𝘢𝘯𝘥 𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴 𝘪𝘯𝘢𝘲𝘶𝘰𝘴𝘰𝘳𝘶𝘮 76-1. 

📌 The research team identified these 𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴 strains from commercial feed additives and then isolated the AMPs they produced using a series of purification techniques. They used advanced LC-MS/MS technology to analyze the molecular weight and sequences of the peptides, and conducted tests to gauge the peptides’ antibacterial activity and stability against heat, pH changes, and proteases. 

1. The researchers successfully isolated two antimicrobial peptides from 𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴 strains 9-1 and 76-1. 

2. They determined the peptides’ sequences using Liquid Chromatography–Mass Spectrometry (LC–MS/MS). Peptide-I (from strain 9-1) had the sequence VFLENVLR and peptide-II (from strain 76-1) had the sequence FSGSGSGTAFTLR. 

3. Both peptides showed strong antibacterial activity against 𝘚𝘵𝘢𝘱𝘩𝘺𝘭𝘰𝘤𝘰𝘤𝘤𝘶𝘴 𝘢𝘶𝘳𝘦𝘶𝘴, 𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴 𝘤𝘦𝘳𝘦𝘶𝘴, 𝘢𝘯𝘥 𝘚𝘢𝘭𝘮𝘰𝘯𝘦𝘭𝘭𝘢 𝘦𝘯𝘵𝘦𝘳𝘪𝘤𝘢. 

4. Peptide-I exhibited good resistance to temperature variations, a range of pHs, and proteases. 

5. Peptide-II could retain its antimicrobial properties in environments with a pH higher than 7.0, indicating a degree of resistance to basic conditions. However, its activity reduced at extremely high temperatures 

6. The minimum inhibitory concentrations of the purified peptides were determined, revealing their effective concentrations against different bacteria. 

7. It was concluded that the identified antimicrobial peptides could be potential substitutes for antibiotics in livestock feeding practices, although further studies are needed. 

📌 The two peptides procured from the 𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴 strains displayed strong antimicrobial activity and good stability, which makes them promising candidates as potential substitutes for traditional antibiotics in animal feeds.

📌 The promising results from this study show how the livestock industry can take a step toward sustainable practices by replacing conventional antibiotics with bacterial AMPs. This could contribute to slowing down the problem of antibiotic resistance. However, the authors emphasise on the need for further investigations to assess the safety and efficacy of these AMPs in practical applications. 

Link to the article : https://tinyurl.com/yc4rua7w