๐ก The main aim of this study was to explore the use of antimicrobial peptides designed against the ฮฉ-Loop of Class A ฮฒ-Lactamases, which increases the effectiveness of ฮฒ-Lactam antibiotics.
๐ The researchers carried out a series of experiments including the assessment of enzyme activities, molecular docking analysis, minimum inhibitory concentration determination, and molecular dynamics simulation study to understand the efficacy of designed peptides in enhancing the potency of ฮฒ-Lactam antibiotics.
๐ Molecular docking analysis indicated that specific designed peptides are effective in inhibiting ฮฒ-Lactamase by interacting with key amino acids in the ฮฉ-Loop of Class A ฮฒ-Lactamases.
๐ Assessment of enzyme activities revealed significant inhibitory effects of the peptides on the kinetic behavior of ฮฒ-Lactamases.
๐ The synergistic use of peptides with ฮฒ-Lactam antibiotics significantly decreased the Minimum Inhibitory Concentrations of all the tested antibiotics, implying an increase in antibiotic effectiveness.
๐ Substantial differences in antibiotic susceptibility were observed with the use of peptides in combination with ฮฒ-lactam antibiotics, indicating enhanced antibacterial activity against strains like ๐. ๐ค๐ฐ๐ญ๐ช ๐ข๐ฏ๐ฅ ๐. ๐ฑ๐ฏ๐ฆ๐ถ๐ฎ๐ฐ๐ฏ๐ช๐ข๐ฆ.
๐ There was a considerable shift in the resistance profile of Gram-negative-bacterial strains when co-treated with synthesized peptides and ฮฒ-lactam drugs.
๐ In tests against ๐๐บ๐ค๐ฐ๐ฃ๐ข๐ค๐ต๐ฆ๐ณ๐ช๐ถ๐ฎ ๐ด๐ฎ๐ฆ๐จ๐ฎ๐ข๐ต๐ช๐ด ๐ข๐ฏ๐ฅ ๐. ๐ต๐ถ๐ฃ๐ฆ๐ณ๐ค๐ถ๐ญ๐ฐ๐ด๐ช๐ด, the combination of peptides and ฮฒ-lactam antibiotics made these strains more vulnerable to ฮฒ-lactam drugs.
๐ The use of ฮฒ-Lactam antibiotics in combination with peptides P5-P10 could potentially be an effective strategy in combating bacterial strains that express class A ฮฒ-Lactamases. The peptides reduced ฮฒ-Lactamase activity, thereby restoring the antimicrobial action of ฮฒ-Lactam antibiotics.ย
Link to the article : https://tinyurl.com/7mcnxtuy
