💡 The main aim of this study was to explore the use of antimicrobial peptides designed against the Ω-Loop of Class A β-Lactamases, which increases the effectiveness of β-Lactam antibiotics.
📌 The researchers carried out a series of experiments including the assessment of enzyme activities, molecular docking analysis, minimum inhibitory concentration determination, and molecular dynamics simulation study to understand the efficacy of designed peptides in enhancing the potency of β-Lactam antibiotics.
📌 Molecular docking analysis indicated that specific designed peptides are effective in inhibiting β-Lactamase by interacting with key amino acids in the Ω-Loop of Class A β-Lactamases.
📌 Assessment of enzyme activities revealed significant inhibitory effects of the peptides on the kinetic behavior of β-Lactamases.
📌 The synergistic use of peptides with β-Lactam antibiotics significantly decreased the Minimum Inhibitory Concentrations of all the tested antibiotics, implying an increase in antibiotic effectiveness.
📌 Substantial differences in antibiotic susceptibility were observed with the use of peptides in combination with β-lactam antibiotics, indicating enhanced antibacterial activity against strains like 𝘌. 𝘤𝘰𝘭𝘪 𝘢𝘯𝘥 𝘒. 𝘱𝘯𝘦𝘶𝘮𝘰𝘯𝘪𝘢𝘦.
📌 There was a considerable shift in the resistance profile of Gram-negative-bacterial strains when co-treated with synthesized peptides and β-lactam drugs.
📌 In tests against 𝘔𝘺𝘤𝘰𝘣𝘢𝘤𝘵𝘦𝘳𝘪𝘶𝘮 𝘴𝘮𝘦𝘨𝘮𝘢𝘵𝘪𝘴 𝘢𝘯𝘥 𝘔. 𝘵𝘶𝘣𝘦𝘳𝘤𝘶𝘭𝘰𝘴𝘪𝘴, the combination of peptides and β-lactam antibiotics made these strains more vulnerable to β-lactam drugs.
📍 The use of β-Lactam antibiotics in combination with peptides P5-P10 could potentially be an effective strategy in combating bacterial strains that express class A β-Lactamases. The peptides reduced β-Lactamase activity, thereby restoring the antimicrobial action of β-Lactam antibiotics.
Link to the article : https://tinyurl.com/7mcnxtuy
